Clinical Applications

• Helps Maintain a Healthy Intestinal Microecology*
• Supports the Natural Immune Response*
• Supports Bowel Regularity*
• Supports Lactose Digestion*

Dr. Q’s Probiotic Daily MAXIMUM STRENGTH is a vegetarian, dairy- and gluten-free, four-strain probiotic totaling 100 billion CFU† per capsule. Each vegetarian capsule is sealed in nitrogen-purged aluminum blister packs to serve as protection from factors proven to compromise the stability of probiotics such as heat, moisture, and oxygen. Dr. Q’s Probiotic Daily MAXIMUM STRENGTH provides four researched strains of beneficial bacteria, including the extensively studied HN019 strain of Bifidobacterium lactis. These live microorganisms have proven health benefits and well- established safety, and have been tested for epithelial cell adhesion and/or resistance to low pH.* To further support resistance to low pH and the delivery of microorganisms to the small intestines, Dr. Q Vitamins employs DRcaps TM gastro-resistant capsules. These specially designed, innovative capsules help slow exposure of actives to stomach acid and ensure more targeted release.*

Discussion

Supplementation with probiotics has many mechanisms of action that benefit health, including but not limited to: (1) supporting metabolic activity, such as the production of short-chain fatty acids and vitamins, nutrient absorption, and digestion of lactose; (2) adhering to intestinal epithelial cells to help maintain a healthy balance of organisms in the intestinal tract; (3) helping to establish populations of good bacteria after disruption in balance; (4) supporting immune function; (5) promoting intestinal epithelial cell survival; (6) supporting healthy bowel function; and (7) degrading oxalates.* [1-8]

Common challenges associated with probiotic supplementation are maintaining stability of the organisms during distribution and shelf life and, once taken by a consumer, survival of the organisms as they travel through the digestive tract so that they reach the target tissue (intestines) alive. To help ensure stability, Dr. Q Vitamins packages the Dr. Q’s Probiotic Daily MAXIMUM STRENGTH capsules in sealed, nitrogen-purged aluminum blister packs to serve as protection from factors proven to compromise the stability of probiotics, such as heat, moisture, and oxygen. Careful selection of organisms is another way Dr. Q Vitamins helps ensure stability. Careful organism selection, as performed for Dr. Q’s Probiotic Daily MAXIMUM STRENGTH, is also a critical aspect of supporting digestive survival. To further support resistance to low pH and the delivery of microorganisms to the small intestines, Dr. Q Vitamins employs DRcaps TM gastro-resistant capsules. These specially designed, innovative capsules help slow exposure of actives to stomach acid to promote a more targeted release.*

HOWARU TM (Bifidobacterium lactis HN019) Discovered in 1899, B lactis play a key role in the human microflora throughout a person’s life. Researchers have identified strain HN019 as having excellent probiotic potential based upon its ability to survive the transit through the human gastrointestinal tract, adhere to epithelial cells, and proliferate. [6] B lactis HN019 has been extensively studied, and its safety and effectiveness is well accepted. [7,8] To assess the impact of Bifidobacterium lactis HN019 supplementation on whole-gut transit time in adults, 100 subjects were given daily doses for 14 days of 17.2 billion colony-forming units (CFU), 1.8 billion CFU, or placebo. Decreases in mean whole-gut transit time over the 14-day study period were statistically significant in the high-dose group and the low-dose group, but not in the placebo group. [8] This level of dosing also supported other parameters of healthy GI function, as were self-reported by patient survey. [8] In another study of preschool-age children, supplementing milk for one year with 1.9 x 10 CFU per day B lactis HN019 and 2.4 g/day of prebiotic oligosaccharides supported both healthy iron status and weight gain. [9] In a randomized, double-blind, placebo-controlled human dietary intervention study in elderly subjects (>60 yrs.), supplementary B lactis HN019 resulted in statistically significant increases in the beneficial organisms bifidobacteria and lactobacilli.* [10]

Lactobacillus acidophilus (Lactobacillus acidophilus La-14) This common inhabitant of the human mouth, intestinal tract, and vagina, is also found in some traditional fermented milks (e.g., kefir) and is widely used in probiotic foods and supplements. It has a history of safe human consumption. The L acidophilus La-14 strain is of human origin and has been identified as a type A1 L acidophilus. L acidophilus shows excellent adhesion to human epithelial cell-lines.* [11,12]

Lactobacillus plantarum (Lactobacillus plantarum Lp-115) This bacteria was isolated from plant material and is abundantly present in lactic acid-fermented foods, such as olives and sauerkraut. In vitro studies have shown that L plantarum strain Lp-115 has excellent adhesion to epithelial cell lines. [13] In addition, L plantarum is resistant to low pH conditions and survives the presence of bile at duodenal concentrations.* [13,14]

Bifidobacterium longum (Bifidobacterium longum Bl-05) The B longum Bl-05 strain is well accepted as safe for human consumption. B longum is resistant to low pH and bile salts and is well suited to the intestinal environment.*

Directions
Take one capsule with water daily, or as directed by your healthcare practitioner. Consult your healthcare practitioner prior to use. Individuals taking medication should discuss potential interactions with their healthcare practitioner.

Does Not Contain
Wheat, gluten, soy, animal or dairy products, fish, shellfish, peanuts, tree nuts, egg, ingredients derived from genetically modified organisms (GMOs), artificial colors, artificial sweeteners, or artificial preservatives.

• Supports Healthy Weight Management*
• Promotes Gut Barrier Function*
• Promotes Intake of Less Calories*
• Supports Reduction of Body Fat Mass*

At the turn of the 20th century, Nobel Prize laureate Ilya Metchnikov introduced the concept that bacteria in yogurt can support the neutralization of gut pathogens, which may, in turn, support longevity. Since then, the role of beneficial bacteria in human health has become well established in the literature and continues to evolve. In 2002, the FAO (Food and Agriculture Organization of the United Nations) and WHO (World Health Organization) working group experts established a definition of probiotics as “live strains of strictly selected microorganisms, which, when administered in adequate amounts, confer a health benefit on the host.” Results of clinical studies have suggested a positive effect of supplemental probiotics on a wide range of health concerns, including gastrointestinal issues, allergies, immune support, and weight-management.*[1-7] Epithelial integrity is key to the function of the gastrointestinal tract, and according to in vitro and animal research, disturbance of this integrity appears to be linked to metabolic disorders. To address the potential effects of consuming specific probiotic bacteria to protect epithelial cells from the effects of pathogenic bacteria, Caco-2 cells were treated with cell-free supernatants (CFSs) of four probiotics— Bifidobacterium lactis‡ 420 (B420), Bifidobacterium lactis HN019, Lactobacillus acidophilus NCFM, and Lactobacillus salivarius Ls-33— and by a cell-free supernatant of a pathogenic bacteria Escherichia coli (EHEC) O157:H7. Tight junction integrity, as well as expression of cyclooxygenases, were measured with results suggesting that the B420 CFS counteracted the damage done by the EHEC CFS.[8] One cannot be certain that B420 CFS would prevent junction damage by live bacteria EHEC, but the results suggest that probiotic bacteria produce soluble metabolites that appear to protect epithelial cells and positively influence cytokine balance. Awareness of the existence and activity of soluble bacterial metabolites in vitro leads to speculation whether the same metabolites are also formed in the intestine with similar effects in vivo. However, the evidence presented does seem to suggest that oral consumption of B420 probiotic may benefit epithelial integrity.* In animal research, the potential benefit of B420 in reducing high-fat, diet-induced body weight gain and diabetes was studied with results indicating a reduction of fat mass and glucose intolerance in both obese and diabetic mice. Reduced intestinal mucosal adherence and decreased levels of plasma lipopolysaccharide suggested a mechanism related to reduced translocation of gut microbes.*[9] HOWARU® Shape B420 (Bifidobacterium animalis subsp lactis B420) The in vitro and experimental animal studies discussed above-utilized B420 with positive results in improving epithelial integrity and potential for a role in weight management. These results led to the need for a human trial to validate these effects. In a double-blind, placebocontrolled study in overweight volunteers (N = 225) aged 18-65, the effects of B420 and the combination of B420 with a prebiotic fiber on body-fat mass and other weight-related parameters were investigated. The authors speculated that the underlying mechanism for reduction of body fat mass could have been related to circulating zonulin (a potential marker of gut barrier function) and C-reactive protein (CRP), speculation that had been supported by previous findings with experimental animals. Subjects were not required to make changes in dietary or exercise habits. Body composition was monitored with dual-energy X-ray absorptiometry, and the primary outcome was a relative change in body fat mass when treatment groups were compared to placebo. Other outcomes included anthropometric measurements, food intake, and blood and fecal biomarkers. Although results indicated that B420 in combination with the prebiotic offered the most significant benefits when compared to placebo (4.5% reduction in body fat mass), B420 alone (1010 CFU/day) resulted in reduced body fat mass of 4.0% with changes in fat mass being most pronounced in the abdominal region and in waist circumference. Significantly reduced caloric intake was also shown in both groups compared to placebo, and there were no differences between groups in the incidence of adverse events.*[7] Building on the data reported for B420 thus far, investigators looked into whether changes in the gut microbiota were associated with clinical benefits and obesity-related issues. Fecal and plasma samples obtained from a subset (n = 134) of the participants in the above mentioned clinical trial were assessed at baseline, two, four, and six months as well as one-month post-intervention. Consumption of B420 resulted in alterations of the specific gut microbiota Lactobacillus and Akkermansia muciniphila. Akkermansia muciniphila, often in lower amounts in obese individuals with metabolic dysfunction, is associated with improved metabolic health. This strain supports gut barrier function and improves obesity-related markers and thus supports the probiotic benefits of B420.[10] More clinical trials are warranted to confirm these effects in larger participant populations and to further elucidate the underlying mechanisms.*

Clinical Applications

• Supports a Healthy Microbiome*
• Replenishes Good Bacteria in the Gut*
• Supports the Natural Immune Response*
• Supports Lactose Digestion*
• Supports Bowel Regularity*

Probiotic Ultra Max 350 Billion features a diverse blend of HOWARU® and FloraFIT ® probiotic strains to support a healthy gut microbiome. Each strain is well-researched and identity-verified and has been genetically characterized and properly classified for your safety and assurance. These strains were not only selected for their health benefits and complementary actions but also for their viability and stability. To protect the bacteria from light and moisture, each flavoring- and sweetener-free serving of Probiotic Ultra Max 350 Billion comes in an opaque, foil-lined stick pack.*

Discussion

Multiplicity of microbiota species is characteristic of the GI microbiome in healthy individuals, and a loss in species has adverse effects.Dietary diversity and taking a variety of probiotic species and strains are methods that help support a healthy microbiome.[1,4] Probiotic Ultra Max 350 Billion provides 18 different strains belonging to 13 different species of three different genera. The HOWARU and FloraFIT strains that comprise this formula are stable, proven, and trusted worldwide.*

Featured Strains: Bifidobacterium lactis HN019 and Lactobacillus rhamnosus HN001

B lactis HN019 and L rhamnosus HN001 have been studied extensively in vitro and in vivo (in animals and humans) to establish beneficial effects and safety.[5-20] In human research, B lactis HN019 significantly decreased mean whole-gut transit time in adults compared to placebo.[8] Supplementation also had a positive effect on functional GI symptoms, as were self-reported by patient surveys. In a randomized, double-blind, placebo-controlled human dietary intervention study in subjects 60 or more years of age, supplementary HN019 resulted in desirable changes in the intestinal microflora, including significant increases in bifidobacteria and lactobacilli.[13] HN019 has also shown promising effects on parameters of cardiovascular and metabolic health. Compared with baseline and control group values, individuals taking 27 billion CFU had a significant reduction in body mass index, significant improvements in cholesterol and low-density lipoprotein metabolism, and significant decreases in tumor necrosis factor-alpha and interleukin-6.[19] In a three-week study, individuals consuming low-fat/low-lactose milk supplemented with HN019 or HN001 showed increased immune activity when compared to a milk-alone run in.[10] These increases were significantly correlated with age; that is, subjects older than 70 years experienced significantly greater improvements than those under 70 years. Researchers suggest that these results demonstrate the ability of HN019 and HN001 to combat some of the deleterious effects of immunosenescence on cellular immunity.[10] Animal studies demonstrate the ability of HN001 to support natural and acquired immunity as well as promote resistance.[17,18] More recently, human studies have demonstrated HN001’s protective effect on skin health in genetically susceptible individuals.*[7,11]

Other Strains in Probiotic Ultra Max 350 Billion Have Demonstrated Probiotic Effects in Either In Vitro or In Vivo Research:

• Cause the development of inhibition zones around Clostridium difficile, Salmonella typhimurium, Staphylococcus aureus, Escherichia coli, and Listeria monocytogenes in agar assays*[20]
• Populate vaginal tissue*[21]
• Induce moderate maturation and activation of dendritic cells*[22]
• Degrade oxalates*[23]
• Increase IgA and IgG responses*[25]
• Reduce levels of E coli and enterococcus after antibiotic therapy*[26]
• Help increase and recover numbers of intestinal lactobacilli and/or bifidobacteria*[27]
• Promote well-being in relation to intestinal cytokine production*[28,29]
• Improve gastrointestinal health and well-being when under challenge*[27,29]
• Help maintain upper respiratory health*[30]
• Alter the fecal microbiota in obese subjects*[31]
• Maintain healthy fluid balance and integrity of intestinal wall*[32]
• Support intestinal barrier function*[32]

Genetic Testing, Acid and Bile Tolerance, Adhesion

Each of the lactic acid-producing strains in Probiotic Ultra Max 350 Billion has a history of safe consumption and, based on testing,

is regarded to have excellent probiotic potential.[20,33,34] Each bacterium has been genetically characterized and properly classified by

independent labs. The organisms have demonstrated tolerance to low pH conditions (hydrochloric acid and pepsin at pH3 for one hour at 37°C), tolerance to bile at concentrations existing in the duodenum, and the ability to adhere to human epithelial cell lines (Caco-2 and HT-29). Adhesion is thought to be an important factor affecting these bacteria’s length of time in the intestines, their ability to modulate immune function, and their ability to take up intestinal “real estate” in competition with other organisms.*[34]

Many of the strains have had additional studies performed, which include testing for the following[20]:

Competitive Inhibition – The ability of probiotics to maintain a healthy microbial balance in the gut is unquestionably important to its usefulness. Probiotics produce inhibitory compounds that limit the growth of other organisms, they compete with other organisms for adhesion sites and nutrients, and they inhibit the production of toxins, among other actions.*

Cytokine and Immune Modulation – Microbiota are key elements in the body’s defense system. Cytokines regulate immune system function, and the ability of many of the strains in Probiotic Ultra Max 350 Billion to upregulate or downregulate various cytokines has been demonstrated.*

Antibiotic Resistance – Antibiotic susceptibility patterns are an important means of indicating the potential of an organism to be readily inactivated by antibiotics used in human therapy. Importantly, acquired antibiotic resistance or antibiotic resistance transfer have not been detected in any of these strains.*

Directions

Dissolve the contents of one stick pack (350 billion CFUs‡)†† into 1-2 oz of pure water and consume once daily, or take as directed by your healthcare practitioner. Consult your healthcare practitioner prior to use. Individuals taking medication should discuss potential interactions with their healthcare practitioner. Do not use if tamper seal is damaged.

Does Not Contain

Wheat, gluten, soy, animal or dairy products, fish, shellfish, peanuts, tree nuts, egg, artificial colors, artificial sweeteners, or artificial preservatives

Symptoms of Adrenal Stress

• Chronic stress and stress-related health problems?
• Fatigue or lack of energy?
• Muscle and join pain or weakness?
• Insomnia, sleep disturbances or difficulty waking?
• Poor memory or difficult retaining information?
• Irritability or mood swings?
• Frequent or chronic infections?
• Increased waist-to-hip ratio or abdominal obesity?
• Low body temperature?
• Low sex drive?

If you answered “yes” to any of these questions, you may beneficent from Adrenal Support.

Clinical Applications

• Supports Relaxed Mood
• Supports Inhibitory Neurotransmitter and Second Messenger Functions
• Supports Neurotransmitter Balance and Neuronal Stabilization
• Supports Healthy Blood Pressure Levels Already Within Normal Range
• Addresses Brain Osmotic Regulation, Glial Cell Function, and Effective Neuronal Transmission

Dr. Q’s Calm Mind is an innovative powdered drink mix. It contains a blend of ingredients that supports the body’s natural synthesis of catecholamines, the inhibitory neurotransmitter GABA, hormonal balance, and healthy glucose metabolism. Dr. Q’s Calm Mind aims to promote a calm, relaxed, well-balanced emotional and physiological state.

Discussion

Inositol Present as the distinct isomer myo-inositol, inositol is a six-carbon cyclic polyalcohol that occurs naturally in all living cells. Fruits, beans, grains, and nuts contain some inositol; however, an 1800-2500–calorie daily diet has been shown to provide only 225-1500 mg of myo-inositol. Of the nearly 100% of ingested myo-inositol that is absorbed in the gastrointestinal tract, more than half becomes lipid bound. In contrast to low plasma concentration, the peripheral nerves have an extraordinarily high concentration of myo-inositol. [1] Inositol is a precursor for the second-messenger phosphatidyl-inositol system, which affects mood status differently than precursors for neurotransmitters. [2] Based upon validated scoring procedures, double-blind, controlled, random-order crossover clinical trials using up to 18 g of myo-inositol per day for a month have demonstrated effectiveness with minimal to no side effects.

GABA (gamma-aminobutyric acid) GABA is an amino acid manufactured in brain cells from glutamate. This primary neurotransmitter, abundant in the cerebral cortex, increases the production of alpha waves (related to a relaxed, yet mentally focused state) while decreasing beta waves (associated with hyperactivity, nervousness, and fleeting thoughts). Sufficient GABA results in the smooth, calming, regular rhythmic flow of electrical impulses in the brain needed for emotional well-being. [5] Supplementation in humans has shown support for the maintenance of healthy cortisol and secretory IgA levels while under stress.

Taurine (2-aminoethanesulfonic acid) Taurine exists mainly in free form in the intracellular space of tissues. This conditionally essential amino acid maintains cell volume via osmoregulation, which is the process that corrects excessive or insufficient concentrations of electrolytes. Taurine also stabilizes cell membranes in the heart and brain—two electrically active tissues. Considered neuroprotective, taurine modulates the ability of mitochondria to buffer intracellular calcium during glutamate depolarization and excitotoxicity (the means by which neurons are overstimulated and damaged) and, thereby, may prevent cell death. [7] In addition to its antioxidant and cytokine-balancing functions, taurine is important to neurotransmission, neuroregulation, and cardiac function. [8,9] Taurine supplementation also increases GABA.

L-Theanine (N-ethyl-L-glutamine) L-Theanine, provided as Suntheanine ® , is protected by more than 40 US and international patents for its various physiological efficacies and L-isomer-specific production processes. A naturally-occurring, biologically active, free-form amino acid, L-theanine gives green tea its characteristic taste. Although notable for its relaxation support, L-theanine may also support nerve health and cognition. Theanine lowers glutamate levels by preventing transport of glutamate’s precursor, glutamine. [10] It may also inhibit neurotransmission, cause inhibitory neurotransmission via glycine receptors, and thereby reduce neuronal overstimulation. [11] L-theanine’s ability to relax the mind without inducing drowsiness has been documented by an increase in alpha wave activity during EEG recording.

Magnesium Sometimes referred to as the relaxation mineral and mainly found in the brain, bones, and muscles, magnesium assists in the transmission of nerve impulses and is essential to more than 300 enzymatic reactions in the body. Magnesium supplementation has been shown to support a healthy mood, including during the menstrual cycle when mood changes are common.

More than one of the ingredients in Dr. Q’s Calm Mind may support a healthy body weight and healthy hormone, lipid, insulin, and glucose metabolism.

Does Not Contain

Wheat, gluten, yeast, soy protein, animal or dairy products, fish, shellfish, peanuts, tree nuts, egg, ingredients derived from genetically modified organisms (GMOs), artificial colors, artificial sweeteners, or artificial preservatives.

Directions

Dissolve one scoop of Dr. Q’s Calm Mind into 6 fl ounces of cool, pure water. Drink one to two times daily, or as directed by your healthcare practitioner.

Consult your healthcare practitioner prior to use. Individuals taking medication should discuss potential interactions with their healthcare practitioner. Do not use if tamper seal is damaged.